Free Radicals and Antioxidant Status in Chronic Osteomyelitis Patients: A Case Control Study
Published: April 1, 2015 | DOI: https://doi.org/10.7860/JCDR/2015/.5781
Abha Jyoti, Saurababh Singh, Bedabrata Mukhopadhyay,Roshni Gavel, Surendra Pratap Mishra
1. Assistant Professor, Department of Biochemistry, Banaras Hindu University Varanasi, Uttar Pradesh, India.
2. Associate Professor, Department of Orthopaedics, Banaras Hindu University Varanasi, Uttar Pradesh, India.
3. Resident, Department of Biochemistry, Banaras Hindu University Varanasi, Uttar Pradesh, India.
4. Resident, Department of Biochemistry, Banaras Hindu University Varanasi, Uttar Pradesh, India.
5. Assistant Professor, Department of Biochemistry, Banaras Hindu University Varanasi, Uttar Pradesh, India.
Correspondence
Dr. Surendra Pratap Mishra,
Assistant Professor, Department of Biochemistry, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
E-mail: drsurendram2@gmail.com
Introduction: Osteomyelitis (OM) is a local or generalized infection of the bone and bone marrow which may be multifactorial in its causation. Chronic infection is characterised by sequestrum and involucrum formation.
Aim: The present study has been carried out for assessing the oxidative stress in chronic OM by measurement of serum oxidants {such as malondialdehyde (MDA), protein carbonyl (PC), nitrite} and the serum antioxidants {such as ascorbic acid, superoxide dismutase (SOD), ceruloplasmin (Cp), blood glutathione} by spectrophotometric method.
Materials and Methods: This was a case control study. About 5 ml of venous blood was collected for the estimation of biochemical parameters. This study comprised of 50 OM patients diagnosed at SSLH Hospital, Varanasi and 50 healthy ages (15-35 y) and sex matched individuals.
Results: Significantly increased (p<0.0001) levels of serum oxidants and significantly decreased (p<0.0001) levels of all serum antioxidants except ceruloplasmin indicated significantly increased (p<0.0001) levels in response to infections in chronic OM patients as compared to the healthy controls.
Conclusion: These results suggest that there occurs an imbalance between oxidants and antioxidants, especially an increase in oxidative stress, as measured by the levels of the parameters: serum MDA, serum protein carbonyl and serum nitrite.
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